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NusA forms a complex with Nun and BOXB. Lane numbers indicate fractions eluted from a Sephadex G-100 column loaded with (A) Nun, NusA, and 32P-BOXB, (B) NusA and 32P-BOXB, and (C) Nun and 32P-BOXB. (Upper) Coomassie blue-stained SDS 16% polyacrylamide gels. (Lower) Autoradiograms of 8 M urea 12% polyacrylamide gels.
