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RNAP binds Nun but not T-Nun. Excluded volumes of a Sephadex G-75 column loaded with Nun (lane 1), T-Nun (lane 2), RNAP (lane 3), RNAP + Nun (lane 4), RNAP + T-Nun (lane 5). Lanes 6 and 7, initial amounts of Nun and T-Nun applied to column.
