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Pyridinoline cross-links in SAOS-2 cell layer collagen compared with human bone
RP-HPLC analysis of pyridinoline cross-links in the cell layer collagen showed HP alone (upper panel) in contrast with the ratio of about 2:1 for HP:LP typical of adult human bone (middle panel). This result indicates that the triple-helical domain cross-linking lysines were fully hydroxylated in the SAOS-2 collagen, whereas in bone collagen they are only partially hydroxylated.
