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. Author manuscript; available in PMC: 2007 Jul 5.
Published in final edited form as: Bone. 2007 Jan 25;40(5):1343–1351. doi: 10.1016/j.bone.2007.01.011

Figure 5.

Figure 5

Co-RT-PCR survey of LH1 mRNA levels in human tissues

LH1 and G3PDH RNAs were co-amplified from fetal human tissues, using dCT32P labelling conditions.

a) Products were separated by electrophoresis on 6% polyacrylamide gels, and detected by autoradiography.

b) Values represent the LH1 mRNA levels normalized to G3PDH as internal standard, as determined by quantitative scans of each lane. Large differences of LH1 mRNA levels were seen between the tissues, with particularly high levels in cartilage and muscle. None, however, exhibited levels approaching that of the SAOS-2 cells.