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. Author manuscript; available in PMC: 2007 Jul 5.
Published in final edited form as: Bone. 2007 Jan 25;40(5):1343–1351. doi: 10.1016/j.bone.2007.01.011

Figure 6.

Figure 6

a: Northern Blot analysis of LH1 mRNA in SAOS-2 cells and skin

5 μg of SAOS-2, human fetal skin and RCS-LTC chondrocyte cell line total RNA was resolved on a 1.2% agarose-formaldehyde gel, blotted to nitrocellulose membrane, probed with a 32P-labelled LH1 cDNA, and detected by autoradiography. RNA kilobase markers are shown at right.

b: Quantitation of LH1 mRNA in SAOS-2 cells and skin by RNAase protection.

RNA was hybridized to a 32P-labelled LH1 antisense probe, digested with nuclease to remove the non-homologous portion of the probe, resolved on a denaturing 6% acrylamide gel, and detected by autoradiography. Lane 1, Full-length probe prior to nuclease digestion. Lanes 2-7, Control LH1 RNA reactions containing 1000, 300, 100, 30, 10, and 3 pg LH1 RNA respectively. Lanes 9-11, Replicate reactions containing 10 μg SAOS-2 RNA. Lanes 14 and 15, Replicate reactions containing 10 μg human fetal skin RNA. Lane 8 and12, Probe only. Lanes 13 and 16 are empty.