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. Author manuscript; available in PMC: 2007 Sep 1.
Published in final edited form as: Dev Biol. 2006 Oct 24;303(1):1–15. doi: 10.1016/j.ydbio.2006.10.026

Figure 2. Gut NCSCs rarely form noradrenergic neurons in sympathetic ganglia.

Figure 2

Chicks were injected in hindlimb bud somites with E14.5 rat gut p75+α 4+ NCSCs. Sequential sections bracketed by sections containing Stmn2+ rat neurons (A, C, E) were hybridized with in situ probes against TH (B), Gata2 (D), and VAChT (F). The number of cells per section that were positive for each marker was counted and expressed as a percentage of Stmn2+ cells in the flanking sections (G). Out of nine chicks examined, one embryo (A, B) contained TH+ cells (B, arrow) adjacent to a cluster of Stmn2+ cells (A). A second embryo (C, D) contained Gata2+ (D, arrow) cells adjacent to Stmn2+ cells (C, arrow). A small percentage of rat cells that engrafted in sympathetic ganglion thus expressed some markers of noradrenergic differentiation, but we did not detect any adjacent sections that were positive for both TH and Gata2. Some of the neurons that failed to express TH did express VAChT (E, F, G) though these cells also did not fully adopt an autonomic fate as we also failed to detect Gata2 in adjacent sections (G; not shown). Bars represent mean ± standard deviation.