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. Author manuscript; available in PMC: 2007 Sep 1.
Published in final edited form as: Dev Biol. 2006 Oct 24;303(1):1–15. doi: 10.1016/j.ydbio.2006.10.026

Figure 3. Fetal gut NCSCs engraft much more extensively than sciatic nerve NCSCs after transplantation into the chick gut.

Figure 3

E14.5 p75+α 4+ gut and sciatic nerve NCSCs were isolated by flow-cytometry from rats ubiquitously expressing hPAP and injected into the cecum of embryonic day 5 (stage 27–28) chick guts, which were then grafted onto the chorioallantoic membrane of 10-day-old chicken embryos and allowed to develop in ovo for a further 5 days. Engraftment was initially assessed chromogenically by reacting every fifth section with the substrate for alkaline phosphatase (purple, A,B). To count the number of engrafted cells, sections adjacent to those positive for chromogenically-detected hPAP were stained with an anti-hPAP antibody and counted based on immunofluorescence. An approximately equal percentage of guts engrafted with gut NCSCs (A; 55%) and sciatic nerve NCSCs (B; 43%), but the number of engrafted sections per gut and the average number of rat cells per section were much higher for gut NCSCs (C). Arrow points to the lumen of the gut. * indicates p < 0.05.