Table 1.
NCSCs migrate extensively and give rise to neurons and glia in diverse locations throughout the developing PNS.
| Location | Marker | Gut (p75+α 4+) | Sciatic nerve (p75+α 4+) | ||||
|---|---|---|---|---|---|---|---|
| chicks engrafted/chicks analyzed | Avg. engrafted sections/engrafted chick | Avg. cells/engrafted section | chicks engrafted/chicks analyzed | Avg. engrafted sections/engrafted chick | Avg. cells/engrafted section | ||
| Dorsal root ganglion | hPAP
N (Stmn2) G (P0) |
3/5
5/37 1/37 |
2.3 ± 1.2
1.2 ± 0.4 1.0 |
ND
1.8 ± 0.8 3.0 |
7/11
0/11 0/11 |
4.6 ± 2.5
0.0 ± 0.0 0.0 ± 0.0 |
ND
0.0 ± 0.0 0.0 ± 0.0 |
| Sympathetic chain | hPAP
N (Stmn2) G (P0) |
5/5
32/37 3/37 |
6.2 ± 3.6
5.1 ± 2.7 2.3 ± 1.5 |
ND
5.7 ± 3.8 2.6 ± 1.6 |
11/11
2/11 4/11 |
7.1 ± 4.0
1.0 ± 0.0 1.5 ± 0.6 |
ND
1.0 ± 0.0 1.5 ± 0.6 |
| Peripheral Nerve | hPAP
N (Stmn2) G (P0) |
5/5
34/37 16/37 |
12.4 ± 3.1
4.5 ± 2.6 2.4 ± 2.0 |
ND
10.4 ± 7.9 6.6 ± 5.2 |
11/11
4/11 11/11 |
15.3 ± 4.5
1.0 ± 0.0 4.5 ± 3.1 |
ND
2.0 ± 1.2 4.1 ± 3.3 |
| Remak’s ganglion | hPAP
N (Stmn2) G (P0) |
4/5
35/37 2/37 |
17.5 ± 10
10.6 ± 6.6 1.0 ± 0.0 |
ND
2.9 ± 1.5 3.0 ±1.4 |
10/11
7/11 0/11 |
19.2 ± 9.3
5.6 ± 5.3 0.0 ± 0.0 |
ND
1.6 ± 0.7 0.0 ± 0.0 |
| Pelvic plexus | hPAP
N (Stmn2) G (P0) |
1/5
20/37 7/37 |
10.0
3.2 ± 1.8 1.9 ± 0.9 |
ND
12.2 ± 9.3 6.1 ± 5.2 |
1/11
1/11 0/11 |
1.0
1.0 0.0 ± 0.0 |
ND
13.0 0.0 ± 0.0 |
| Gut | hPAP
N (Stmn2) G (P0) |
1/5
20/37 9/37 |
4.0
3.9 ± 3.3 2.2 ± 1.2 |
ND
3.2 ± 2.1 2.4 ± 1.4 |
0/11
0/11 0/11 |
0.0 ± 0.0
0.0 ± 0.0 0.0 ± 0.0 |
ND
0.0 ± 0.0 0.0 ± 0.0 |
| Dermis | hPAP
N (Stmn2) G (P0) |
2/5
0/37 0/37 |
4.5 ± 3.5
0.0 ± 0.0 0.0 ± 0.0 |
ND
0.0 ± 0.0 0.0 ± 0.0 |
11/11
0/11 0/11 |
10.2 ± 8.6
0.0 ± 0.0 0.0 ± 0.0 |
ND
0.0 ± 0.0 0.0 ± 0.0 |
| Ventral root | hPAP
N (Stmn2) G (P0) |
2/5
1/37 14/37 |
4.0 ± 0.0
1.0 2.8 ± 2.9 |
ND
1.0 4.0 ± 3.0 |
2/11
0/11 1/11 |
4.0 ± 4.3
0.0 ± 0.0 1.0 |
ND
0.0 ± 0.0 8.0 |
| Ventral column of the neural tube | hPAP
N (Stmn2) G (P0) |
3/5
0/37 30/37 |
3.0 ± 3.5
0.0 ± 0.0 4.5 ± 3.8 |
ND
0.0 ± 0.0 4.1 ± 2.3 |
0/11
0/11 0/11 |
0.0 ± 0.0
0.0 ± 0.0 0.0 ± 0.0 |
ND
0.0 ± 0.0 0.0 ± 0.0 |
Uncultured E14.5 rat sciatic nerve or gut NCSCs were isolated by flow cytometry and injected into the hindlimb bud somites of stage 17–18 chick embryos. In some chicks, the progeny of hPAP-expressing NCSCs were detected by a chromogenic reaction for alkaline phosphatase activity to assess the engraftment of transplanted cells in a way that was unbiased by cell fate. In other chicks, the engraftment and fate of rat cells were determined by in situ hybridization using rat-specific probes against the neuronal marker Stmn2 and the glial marker P0. Statistically significant differences (using a Bonferroni corrected p-value to account for multiple comparisons; p<0.001) in the engraftment of gut and sciatic nerve NCSCs are indicated with the higher value underlined. All statistics represent mean±SD. ND means not determined.