Figure 4. Primary immature cortical neurons isolated from 12-lipoxygenase knock out mice are resistant to glutamate-induced death.
Murine primary immature cortical neuronal cells (C57BL/6, A; B6.129S2-Alox15 tm1Fun, B) were challenged with glutamate (10mM) for 24 h. Cell viability was assessed by lactate dehydrogenase assay. Treatment specifications are described in legend of Figure 1. α-tocotrienol, 100 nM. †, lower compared to the corresponding control non-treated group, also lower compared to corresponding group in 12-lipoxygenase deficient neurons; *, higher compared to the corresponding toxin-treated group. P<0.05.