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. Author manuscript; available in PMC: 2008 May 11.
Published in final edited form as: Brain Res. 2007 Feb 2;1145:1–10. doi: 10.1016/j.brainres.2007.01.119

Figure 1. cAMP induces neurite outgrowth and produces differential regulation of CHT and the cholinergic locus expression in NSC-19 cells.

Figure 1

(A) Phase-contrast microscope pictures were taken after 36 h of treatment. Objective lens 20 x. Size bars: 100 μM. (B) ChAT activity was assayed in cell extracts as described in Experimental Procedures. Data are shown in the bar graph as means ± SEM of two independent cultures (each experiment was performed in duplicate). (*) significantly different from corresponding untreated control. (C) Total RNA preparations were subjected to RT-PCR reactions with CHT, ChAT and VAChT primers. RT-PCR with β-actin primers served as a loading control. PCR product band intensities were quantified by the Kodak Image Station Software. Results are expressed as means ± SEM of three experiments. (*) significantly different from corresponding untreated control.