Figure 5.

ORF essentiality predictions using iAF1260. This heat map characterizes the agreement between ORFs predicted to be essential using iAF1260 and those experimentally determined from Baba et al (2006) and Joyce et al (2006). The enlarged region details how each a row corresponds to a computationally predicted essential ORF (188 total). The overall agreement between iAF1260 predictions and those found to be experimentally essential (overall, column 1) is shown along with a breakdown for ORFs found to be essential under rich media conditions (rich, column 2), under both glucose and glycerol minimal media conditions (shared, column 3) and under just glucose minimal medium conditions (glucose, column 4). ORFs are further grouped by their COG functional class (see Figure 1 for abbreviations; MU-ORF belongs to multiple COG classes). Dark blue indicates the condition under which each ORF was found to be essential. For example, folP was predicted to be an essential ORF for the biosynthesis of folate in iAF1260 under these conditions, but was not identified as essential by Baba et al (2006). This suggested the possibility of an alternative pathway for this step in E. coli that has yet to be characterized.