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. 1997 Jan;71(1):818–823. doi: 10.1128/jvi.71.1.818-823.1997

Efficient multiplication of a Semliki Forest virus chimera containing Sindbis virus spikes.

J Smyth 1, M Suomalainen 1, H Garoff 1
PMCID: PMC191124  PMID: 8985423

Abstract

Using the Semliki Forest virus (SFV) and Sindbis virus (SIN) cDNAs we have constructed recombinants in which the spike genes were exchanged. Analyses of expression showed that the SFV/SIN(spike) RNA directed efficient assembly of infectious virus, whereas the reciprocal SIN/SFV(spike) RNA was completely unable to assemble virus. This was apparently due to a defective capsid-spike interaction.

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Selected References

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