Figure 2.

Immunoblot of representative nuclear (N), membrane (M), and cytoplasmic (C) fractions of HEK-293T cells transfected with vector only (WT) or with menin. Fifty micrograms of protein was loaded for each fraction for the menin blot in WT cells, 5 μg of protein was used for the menin blot in menin-transfected cells, 25 μg of protein is present in each lane for the nuclear pore protein blot, and 12.5 μg of protein was used per lane for the tubulin blot. The blots shown for tubulin and for nuclear pore protein were from WT cells, but blots of fractions from menin-transfected cells gave comparable results.