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Optimization of the 5'-proximal region of ACHE-S is critical but not sufficient for high expression levels. The following constructs were transiently expressed by A. tumefaciens-infiltration of N. benthamiana leaves: hACHE-S, o-hACHE-S, h-oACHE-S, and oACHE-S. Averages of AChE activity of 6 replicates were normalized to that of hACHE-S to obtain the fold increase values (+ SEM) shown.
