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. 2007 Jul 6;3(7):e132. doi: 10.1371/journal.pcbi.0030132

Figure 3. Neighbor Cluster spy0127–0130 .

Figure 3

(A) Schematic representation of the spy0127–0130 cluster. Gene size (bp) is indicated under the gene name, and the position of primers for the reverse transcription of streptococcal mRNA are indicated by colored arrows (forward primers, F; reverse primers, R). Eight different combinations of primers were used, and the expected sizes of the eight resulting cDNAs that would be produced if genes compose an operon are indicated in the numbered, colored boxes. cDNA box colors correspond to colors of the primer pairs that would generate each fragment.

(B) A 1% agarose gel of the cDNA fragments amplified from mRNA with each primer combination. Lane numbers correspond to the numbering of the predicted cDNAs from (A). First lane of gel (MW) contains 1 kb Plus DNA ladder, and the sizes of the relevant DNA fragments in the ladder are indicated (bp).