(A) SNR is inversely proportional to the square-root of the measurement bandwidth fLP, and proportional to Simulated step detection threshold contours (SNR = 4) as a function of FC for stepping rates between 10 s−1 and 1000 s−1, and (B) for persistence lengths between 1 nm and 100 nm. Contours for three step lengths are shown, ΔL = 0.3 nm (triangles), ΔL = 0.9 nm (squares), and ΔL = 1.5 nm (circles). Solid lines indicate step detection thresholds calculated from the exact formula, Eq. 10, and dashed lines indicate the SNR threshold calculated from the approximate formula, Eq. 25. L = 1000 nm, d = 1000 nm, in (A) Lp = 50 nm, and in (B) kcat = 100 s−1. In panel B, the dotted line indicates the border between the high-force (III) and the medium-force (II) regions.