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The C=C stretch regions of the resonance Raman spectra of reconstituted spheroidene in the Rb. sphaeroides R26 photosynthetic reaction center. (a) NA, (b) 15′-2H, (c) 15,15′-2H2, (d) 13-13C, (e) 13,14-13C2, (f) 10-2H, (g) 10,12-2H2, and (h) 12,14-2H2. Calculated frequencies have been indicated above each spectrum by bars. Values calculated for the 15,15′-cis structure are displayed in solid representation and values for the 13,14-cis structure in shaded representation.
