Figure 3. CML-collagen inducedCollagen stimulates apoptosis is mediated through RAGE.
Primary cultures of human adult osteoblastic cells were incubated with CML-collagen (200μg/ml) in presence or absence of antibody specific for the extra-cellular domain of RAGE (10 μg/ul) or non-immune serum (10 μg/ul) for 24 hours. In some cases cells were incubated with antiserum or non-immune serum alone. The extent of apoptosis was determined by ELISA. Each value represents the mean of 4 replicates ± SEM. The experiment was performed three times with similar results.