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. Author manuscript; available in PMC: 2008 Feb 1.
Published in final edited form as: Bone. 2006 Oct 24;40(2):345–353. doi: 10.1016/j.bone.2006.09.011

Figure 6. Apoptotic effect of CML-Collagen increased in differentiated osteoblasts.

Figure 6

MC3T3-E1 cells (A) or primary neonatal calvarial osteoblastic cells (B) were cultured in the presence or absence of ascorbic acid (50 μg/ml) for one or two weeks. Cells were then incubated with CML-collagen or unmodified collagen (200 μg/ml) for 24h. Control cells were incubated in assay medium alone. Apoptosis was determined by ELISA and the values were normalized by cell number. Each value represents the mean of 4 replicates ± SEM. The experiment was performed three times with similar results.