FIG. 3.

Manual secondary assays confirm anti-MV activities of the four hit candidates. (A) Quantification of the extent of chemical lysis of cells incubated in the presence of compound. The values reflect the percentages of signal intensity compared with cells incubated in the presence of DMSO. Averages of four replicates are shown, and the error bars represent standard deviations (SDs). (B) Quantification of proliferation activities of cells incubated in the presence of compound. The number of live cells was determined 30 h after compound addition. The values indicate the percentages of live cells compared with DMSO-treated controls. Averages of three experiments are shown, and the error bars represent SDs. (C) Virus yield assay to determine the reduction of virus loads. Cells were infected with MV-Edm in the presence of different compound concentrations, and titers of cell-associated viral particles were determined by TCID₅₀ titration 36 h postinfection. Titers were normalized for DMSO-treated control infections. Average values of two experiments are shown, and the error bars represent standard errors of the mean.