FIG. 5.

Compound 16677 is a well-behaved, target-specific MV inhibitor. (A) Unrelated protein material or variation of the amount of target molecules does not affect 16677 activity. Virus yield-based dose-response curves as described in the legend to Fig. 4B were generated for 16677 upon infection of cells with MV-Alaska in the absence of BSA, in the presence of BSA, and after infection with a fivefold-higher virus inoculum. The values represent average EC₅₀s of two experiments, and the error bars represent standard errors of the mean (SEMs). (B) 16677 is highly MV specific. Virus yield-based dose-response curves were generated for MV, the closely related CDV, and the more distantly related hPIV2. The titers were normalized for DMSO-treated control infections to facilitate comparison of different experiments. Average values of two experiments are shown, and the error bars represent SEMs.