TABLE 1.

Structures of confirmed HTS hits and summary of secondary-assay results

^a Concentration at which a 50% reduction of virus yields (MV-Edm strain) was observed. Averages and standard deviations (SDs) are based on three experiments.

^b Determined for Vero cells using a CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega). Averages and SDs are based on four experiments. The highest concentrations examined were 150 μM for compounds 15960, 68290, and 99920 and 300 μM for compound 16677. The values in parentheses reflect a 50% reduction in the number of viable cells after a 30-hour incubation in the presence of compound, determined using a trypan blue exclusion assay. Averages and SDs are based on three experiments. ND, not determined.

^c SI, selectivity index (CC₅₀/EC₅₀). The values in parentheses are based on CC₅₀s obtained using the trypan blue exclusion assay.