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Determination of Z′ factor for the assay. The signals obtained with 1 μM probe (⧫) and a mixture of 1 μM probe and 7.2 μM receptor (⋄) are shown. DMSO (4%) and Tween 20 (0.01%) were included in the buffer. Experiments were conducted at room temperature on a Spectramax Gemini XPS plate reader in Greiner Bio-one 384-well plates, with 30 μl used per well.
