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. 2007 Apr 23;51(7):2388–2395. doi: 10.1128/AAC.00150-07

FIG. 6.

FIG. 6.

Fluorescence intensity of 2 μM C18-Aib-LY mixed with compounds from the library in Fig. 4 in the presence (gray bars) and absence (white bars) of 6 μM [Fe(II)(env2.0)3]2+. The lower dashed line (Fmin) indicates the fluorescence intensity of the [Fe(II)(env2.0)3]2+-C18-Aib-LY mixture in the absence of compound (20 fluorescence units), and the upper dashed line (Fmax) indicates the fluorescence intensity of 2 μM C18-Aib-LY alone (100% intensity, 80 fluorescence units). Compounds from chemset 12 were aggregators and caused the probe fluorescence to rise above Fmax. The chemset 11 compounds for which the results are shown here and C18-Aib did not aggregate at the concentrations used in the assay. Compounds were diluted in buffer by a factor of 25 from stock solutions in DMSO. The experiments were repeated in duplicate.