FIG. 2.
Dose-dependent inhibition of HBV DNA release from HepG2.2.15 cells 6 days after incubation with 0.1, 0.3, and 0.5 μM 3TC, 0.1, 0.3, and 0.5 μM Hy3TC, and 0.05, 0.1, and 0.3 μM β-l-Hyd4C. HBV DNA was detected in the medium by PCR. Serial dilutions of a cloned HBV genome (GE) served for calibration. For convenient quantification, dilutions of the PCR product from the untreated control corresponding to 100%, 50%, and 20% of the signal were used. “5 ×” means that 5 × 10y GE (5×) were used for PCR.