FIG. 3.

Time-dependent inhibition of HBV DNA release from HepG2.2.15 cells by 3TC, Hy3TC, and β-l-Hyd4C. The cells were treated with the indicated drugs for 3, 6, and 9 days. 3TC was used at 0.3 μM, Hy3TC at 0.5 μM, and β-l-Hyd4C at 0.1 μM. HBV DNA of the supernatants was analyzed by PCR. Serial dilutions of a cloned HBV genome served for calibration of the PCR assay (GE). For convenient quantification, dilutions of the PCR product from the untreated control corresponding to 100%, 50%, and 20% of the signal were used. −, no treatment.