FIG. 8.

PPMOs partially protect mice from WNV disease. Six-week-old C3H female mice were inoculated s.c. in the left rear footpad with 10³ PFU of WNV on day 0. Groups of eight mice were treated i.p. with 100 or 200 μg of PPMOs or the vehicle alone. (A and B) Survival rates among mice treated daily on days 0 to 8 with the 5′-end PPMO and the 3′ CSI PPMO, respectively. The survival curves for groups treated with the vehicle alone and the scramble PPMO are the same in panels A and B and are repeated for ease of comparison. The asterisk indicates that the survival curve for the group treated with the 5′-end PPMO at 200 μg was significantly different from that for the group treated with the scramble PPMO at 200 μg (log rank test; P = 0.04). (C) Average clinical scores for groups treated daily with the indicated PPMOs on days 0 to 8. Clinical scores were assigned a scale of 0 (normal) to 6 (dead or euthanized). The scale of clinical scores is further defined in Materials and Methods. (D) Survival rates of mice treated daily from days 5 to 15 after viral inoculation.