FIG. 3.

Assays for LMV resistance. Replicate cultures of Huh7 cells were transiently transfected with a laboratory clone of wild-type (WT) HBeAg-negative HBV or one of its mutant derivatives. After 5 days of continuous exposure to LMV at the concentrations indicated, intracellular core-associated HBV DNA was isolated, separated by agarose gel electrophoresis, and detected by Southern blotting and autoradiography.