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. 2007 Apr 23;51(7):2313–2323. doi: 10.1128/AAC.01289-06

FIG. 3.

FIG. 3.

IF-S inhibits the binding of Leishmania pifanoi amastigotes to MDDCs and K562-CD209 cells. (A and B) Flow cytometry analysis of the binding of CFSE-labeled L. pifanoi amastigotes to MDDCs in the presence of IF-S or the indicated antibodies. The percentage of cells with bound amastigotes is indicated. MOI, multiplicity of infection. (C and D) DC-SIGN cell surface expression (percentage of marker-positive cells and mean fluorescence intensity) on the MDDCs analyzed and for which the results are shown in panels A and B, as determined by flow cytometry. (E) Flow cytometry analysis of the binding of CFSE-labeled L. pifanoi amastigotes to K562-CD209 cells (multiplicity of infection [MOI], 5:1) in the presence of IF-S or an anti-DC-SIGN antibody. The percentage of cells with bound amastigotes is indicated. (F) Phase-contrast images (left panels) and epifluorescence visualization (right panels) of the experiment whose results are shown in panel E.