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. 2007 Apr 23;51(7):2313–2323. doi: 10.1128/AAC.01289-06

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FIG. 5. — IF-S inhibits DC-SIGN-dependent adhesive functions. (A) Flow cytometry determination of the DC-SIGN cell surface expression on immature MDDCs, K562 cells, and K562-CD209 cells. The percentage of marker-positive cells and the mean fluorescence intensity are indicated in each case. (B) Adhesion of MDDCs, K562 cells, and K562-CD209 cells to recombinant ICAM-3-Fc in the presence of mannan, IF-S, an anti-DC-SIGN antibody (MR1), or an irrelevant antibody. Each condition was assayed in triplicate, and the data shown are the means ± standard deviations. (C) Flow cytometry determination of the DC-SIGN cell surface expression on Jurkat and Jurkat-CD209 cells. The percentage of marker-positive cells and the mean fluorescence intensity are indicated in each case. (D) Adhesion of Jurkat-CD209 cells to recombinant ICAM-3-Fc performed as described in the legend to panel B.