FIG. 7.

Inhibition of PfFAS reaction with triclosan, using C₁₀-ACP as the primer. The reaction was initiated by the addition of 5 μg PfFabB/F, and the first aliquot was taken out at 0 min (A). After 10 min of incubation, another aliquot was taken out (B) and 2 μg PfFabG was added to the same reaction mixture. After incubation for 10 min, another aliquot was taken out (C) and 3 μg PfFabZ was added to the reaction mixture. The mixture was incubated for another 10 min before an aliquot was taken out (D). Finally 2 μg PfFabI plus triclosan was added to the mixture and was incubated for 10 min before termination of the reaction (E). (F) The reaction in which PfFabI was added without triclosan. All the aliquots were snap frozen and analyzed by MALDI-TOF MS. Results are shown for C₁₀-PfACP (9,913 Da), β-keto C₁₂-PfACP (9,955 Da), β-hydroxy C₁₂-ACP (9,957 Da), C₁₂-PfACP (9,941 Da), and C₁₄-PfACP (9,969 Da). The molecular weights of acylated-ACPs were calculated keeping holo ACP (9,758 Da) as the standard.