FIG. 2.
Effect of flow rate on HIV-1 deactivation. Ten milliliters of human plasma containing ∼2 × 105 CCID50 of HIV-1 was added at room temperature to copper oxide-containing filters or to control filters containing no copper oxide. The plasma was eluted from the filters via a peristaltic pump at flow rates of 0.25 ml/cm2/min to 0.025 ml/cm2/min. The eluates were then subjected to sequential fourfold dilutions and added to MT2 cells. The CCID50 was determined after 4 days of culture as previously described (2). The y axis shows the difference between the log10 CCID50s obtained by the eluates of the control filters minus the log10 CCID50s obtained by the eluates of the copper oxide-containing filters. The average and standard deviation of triplicate experiments for each flow rate are shown. *, undetectable residual virus.