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FASI purification
| Stepa | Total protein (mg) | Activity (mU/ml) | Total activity (mU) | Yield (%) | Sp act (mU/mg) | Purification factor (fold) |
|---|---|---|---|---|---|---|
| Lysate | 0.130 | 9.09 | 545 | 100 | 69.9 | |
| (NH4)2SO4 | 0.1088 | 19.5 | 341 | 63 | 179 | 3 |
| Anion | 0.0424 | 30.8 | 208 | 38 | 726 | 10 |
| Gel | 0.0338 | 8.67 | 43.4 | 8 | 257 | 4 |
Aliquots were taken after ultracentrifugation (lysate), dialysis of the pellet from 2.0 M ammonium sulfate precipitation [(NH4)2SO4], anion exchange (anion), and gel filtration (gel).
