TABLE 3.
Properties of genes and gene products encoded by cosmid R+
| Gene product | Gene properties
|
Protein properties
|
Protein correspondence
|
Protein with highest similarity | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Range | % GC | Length (amino acids) | Mol mass (kDa) | pI | % Identity | % Similarity | E value | Reference | ||
| ORF1 | 1-1150 | 68 | 384 | 41.8 | 8.9 | 59 | 70 | 9e-104 | gi 121608751 ref YP_996558.1 | Transposase (Verminephrobacter eiseniae) |
| ORF2 | 1218-2147 | 63 | 309 | 33.3 | 6.0 | 61 | 77 | 1e-94 | gi 118592793 ref ZP_01550182.1 | ABC transporter substrate-binding protein (Stappia aggregata) |
| ORF3 | 2509-3618 | 67 | 369 | 38.9 | 6.3 | 85 | 90 | 1e-95 | gi 56479427 ref YP_161016.1 | Possibly flavin-dependent dehydrogenase (Azoarcus sp. strain EbN1) |
| ORF4 | 3757-4656 | 57 | 299 | 32.9 | 6.2 | 72 | 84 | 8e-121 | gi 56478704 ref YP_160293.1 | Possibly involved in regulation of phenolic degradation (Azoarcus sp. strain EbN1) |
| ORF5 | 5096-5701 | 62 | 201 | 22.7 | 5.3 | 70 | 78 | 1e-68 | gi 89358401 ref ZP_01196223.1 | Conserved hypothetical protein (Xanthobacter autotrophicus) |
| ORF6 | 5712-6680 | 62 | 322 | 36.7 | 6.7 | 61 | 72 | 1e-109 | gi 110634534 ref YP_674742.1 | Putative glutathione S-transferase (Mesorhizobium sp.) |
| RHS | 6763-7659 | 62 | 298 | 33.2 | 7.5 | 55 | 70 | 5e-92 | gi 118698587 ref ZP_01556663.1 | Pyrogallol hydroxytransferase (Burkholderia ambifaria) |
| RHL | 7708-10455 | 63 | 915 | 102.7 | 6.6 | 67 | 66 | 0.0 | gi 118698588 ref ZP_01556664.1 | Pyrogallol hydroxytransferase (Burkholderia ambifaria) |
| BQDHL | 11332-13554 | 65 | 740 | 81.1 | 5.6 | 57 | 73 | 0.0 | gi 119946408 ref YP_944088.1 | Pyruvate dehydrogenase (Psychromonas ingrahamii) |
| BQDHS | 13589-14854 | 69 | 421 | 43.3 | 5.9 | 55 | 70 | 2e-102 | gi 83593214 ref YP_426966.1 | Dihydrolipoamide acetyltransferase (Rhodospirillum rubrum) |
| BQDHM | 14866-16263 | 65 | 465 | 49.6 | 6.2 | 68 | 83 | 3e-180 | gi 23013388 ref ZP_00053288.1 | Pyruvate/2-oxoglutarate dehydrogenase (Magnetospirillum magnetotacticum) |
| ORF7 | 16611-18260 | 68 | 549 | 60.3 | 7.7 | 38 | 59 | 6e-110 | gi 56419244 ref YP_146562.1 | Acetoin operon expression regulatory protein (Geobacillus kaustophilus) |
| ORF8 | 18310-18933 | 61 | 207 | 23.8 | 5.4 | 38 | 53 | 2e-38 | gi 88940881 ref ZP_01146311.1 | Putative protein-disulfide isomerase (Acidiphilium cryptum) |
| ORF9 | 19004-20239 | 65 | 411 | 42.6 | 9.8 | 54 | 69 | 3e-76 | gi 71907514 ref YP_285101.1 | Major facilitator superfamily MFS_1 (Dechloromonas aromatica) |
| ORF10 | 20236-21306 | 63 | 356 | 38.8 | 5.5 | 44 | 58 | 2e-66 | gi 83308699 emb CAJ01609.1 | Cobalamin synthesis protein cobW/p47k family protein (Methylocapsa acidiphila) |
| ORF11 | 21339-21920 | 65 | 193 | 21.8 | 7.1 | 54 | 67 | 2e-38 | gi 91790635 ref YP_551587.1 | Antibiotic biosynthesis monooxygenase (Polaromonas sp.) |
| ORF12 | 21954-23414 | 65 | 486 | 51.9 | 5.6 | 73 | 86 | 0.0 | gi 118744335 ref ZP_01592329.1 | Succinic semialdehyde dehydrogenase (Geobacter lovleyi) |
| BTDHL | 23426-24336 | 68 | 303 | 31.3 | 6.0 | 45 | 63 | 4e-46 | gi 27377791 ref NP_769320.1 | Dehydrogenase (Bradyrhizobium japonicum) |
| BTDHS | 24352-24957 | 60 | 201 | 22.2 | 10.4 | 39 | 53 | 1e-14 | gi 78693872 ref ZP_00858386.1 | Hypothetical protein (Bradyrhizobium sp.) |
| ORF13 | 25135-26388 | 65 | 417 | 46.6 | 5.5 | 48 | 68 | 1e-104 | gi 119946410 ref YP_944090.1 | Peptidase M24 (Psychromonas ingrahamii) |
| ORF14 | 26433-27548 | 61 | 371 | 40.8 | 6.4 | 51 | 67 | 3e-102 | gi 74317595 ref YP_315335.1 | NADH-dependent flavin oxidoreductase (Thiobacillus denitrificans) |
| ORF15 | 27752-29737 | 68 | 661 | 72.8 | 6.9 | 37 | 57 | 3e-117 | gi 114567214 ref YP_754368.1 | Acetoin operon expression regulatory protein (Syntrophomonas wolfei) |