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Binding of oxidized OxyR (OxyRox) and reduced OxyR (OxyRred) to the upstream regions of RSP1408 (A), RSP2638 (B), and RSP3751 (C), as determined by gel mobility shift assays. To generate reduced conditions, 200 mM DTT was added to the binding reaction mixtures. The following amounts of OxyR were added to the reaction mixture: 50 ng (lanes 1 and 4), 100 ng (lanes 2 and 5), and 150 ng (lanes 3 and 6). Lane C contained the control without OxyR.
