FIG. 2.
(A) Footprint assay of oxidized OxyR (OxyRox) and reduced OxyR (OxyRred) for the upstream region of the R. sphaeroides class III gene RSP3751. To generate reduced conditions, 200 mM DTT was added to the binding reaction mixtures. The regions protected by OxyR are indicated by vertical lines on the left; the numbers indicate the binding positions with respect to the translational start site. Lane c contained the control without OxyR. Lanes G, A, T, and C show the sequencing reaction. (B) Sequence of the upstream region of RSP3751. The start of the RSP3715 coding sequence is indicated by an arrow. Binding sites of reduced OxyR are underlined.