TABLE 2.
Functional complementation of M. tuberculosis with fabG homologs
| Strain | Plasmid | Allele | Antibiotic selection | Transformation efficiencya |
|---|---|---|---|---|
| Mess 19 | pUC-Hyg-Int | None | Hygromycin | 10 |
| Mess 19 | pDUCK1 | M. tuberculosis operon | Kanamycin | 1 × 105 |
| Mess 19 | pDUCK2 | M. tuberculosis operon | Hygromycin | 2 × 106 |
| Mess 25 | pDUCK8 | M. tuberculosis fabG1 | Hygromcyin | 1.7 × 105 |
| Mess 26 | pDUCK9 | M. smegmatis fabG | Gentamicin | 1 × 105 |
| Mess 26 | pDUCK15 | E. coli (Ts) | Gentamicinb | 1 |
| Mess 26 | pDUCK16 | E. coli | Gentamicinb | 1 |
| Mess 26 | pDUCK18 | E. coli (Ts) | Gentamicinb | 2 |
a
Transformation efficiencies for the plasmids are expressed as the number of transformants per μg of plasmid DNA.
b
Transformants were isolated at 30°C.