TABLE 2.
Relative binding of WT IHF to WT and mutant ihfA F oriT binding sites
| Construct name | ihfA sequencea | Relative [IHF]1/2b | nc |
|---|---|---|---|
| WT 50-bp | CACGCAAAAACAAGTTTTTGCTGATTTTTCTTTATAAATAGAGTGTTATG | 1.0 ± 0.2 | 3 |
| T172G/T174C/T175G | CACGCAAAAACAAGTTTTTGCTGATgTcgCTTTATAAATAGAGTGTTATG | 4.7 ± 0.4 | 4 |
| WT 40-bp | CAAGTTTTTGCTGATTTTTCTTTATAAATAGAGTGTTATG | 1.0 ± 0.1 | 3 |
| T178C/T179A/A180G | CAAGTTTTTGCTGATTTTTCTcagTAAATAGAGTGTTATG | 3.8 ± 0.7 | 3 |
| WT 46-bp | GTTTTTGCTGATTTTTCTTTATAAATAGAGTGTTATGAAAAATTAG | 1.0 ± 0.04 | 3 |
| T181G/A182C/A183G/A184C | GTTTTTGCTGATTTTTCTTTAgcgcTAGAGTGTTATGAAAAATTAG | 17.8 ± 3.9 | 3 |
| WT 52-bp | GCTGATTTTTCTTTATAAATAGAGTGTTATGAAAAATTAGTTTCTCTTACTC | 1.0 ± 0.1 | 3 |
| T192G/T193G/A194C | GCTGATTTTTCTTTATAAATAGAGTGggcTGAAAAATTAGTTTCTCTTACTC | 14.2 ± 2.4 | 3 |
a
The region most similar to the IHF consensus sequence is shown in bold, and the 5′ A/T-rich region is underlined. Substitutions are in lowercase letters.
b
Relative half-maximal binding reported with standard errors.
c
Number of measurements.