FIG. 3.
Three clones of the wild type (closed circles) and those of the ΔCRP strains (open circles) of T. thermophilus were each grown in a rich medium, and the A600 values at the indicated times are expressed as means ± SD, respectively. Expression of T. thermophilus CRP mRNA in the wild-type strain at the indicated times (open triangles) was investigated using GeneChip technology, as described in Materials and Methods, and expressed as normalized intensity ± SD. The intracellular cAMP level in the wild-type strain at the indicated times (closed triangles) was monitored by means of a cAMP enzyme immunoassay and expressed as pmol per mg protein ± SD.