FIG. 5.
Effect of xcpRZ deletion on the secretory activity of PAQA. The secretory activity of the PAQA subunit was studied in a mutant strain deleted of the xcpRZ operon (ΔRZ). (A) Proteolytic activity, on a TSA-milk plate containing 2 mM IPTG, of the mutant strain bearing pLAFR3, pAX24 (xcp gene cluster), pMMB190, or pMMB-PAQA (PAQA). (B) Immunoblot of cellular and extracellular protein extracts from the same strains described for panel A and probed with LasB antiserum. C, cellular proteins; Ex, extracellular proteins.