TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Escherichia coli strains | ||
| TG1 | supE hsdΔR thi Δ(lac-proAB) F′ (traD36 proAB+laqIqlacZΔM15) | Laboratory collection |
| 1046 | met supE hsdS recA | Laboratory collection |
| BL21(DE3) | F−hsdS gal ompT, lysogen of DE3 carrying T7 polymerase gene | 34 |
| DH5α | supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Invitrogen |
| CC118 (λpir) | Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB arg(Am) recA1 Rfr (λpir) | 17 |
| Pseudomonas aeruginosa strains | ||
| PAO1 | Prototroph, chl-2 | B. Holloway |
| PAO1ΔPQ | xcpPQ deletion mutant | 9 |
| PAO1ΔPAQA | xphA-xqhA deletion mutant | This work |
| PAO1ΔPQPAQA | xcpP xcpQ xphPAxqhQA deletion mutant | This work |
| PAO1ΔRZ | Mutant strain deleted of the xcpRZ operon | V. Chapon |
| Plasmids | ||
| pMMB190 | Broad-host-range vector, Apr, lacUV5 promoter, lacZα | 24 |
| pMMB67HE | Broad-host-range vector, Apr, tac promoter | 14 |
| pRK2013 | ColE1, Tra+ Mob+ Kmr | 10 |
| pCR2.1 | ColE1, f1 ori, Apr Kmr | Invitrogen |
| pCR2.1-PAQA | pCR2-1 bearing xphA and xqhA genes | This work |
| pKNG101 | Smr, oriR6K mobRK2 sabBR+ (suicide vector) | 19 |
| pKNΔPAQA | Mutator plasmid for xphA-xqhA deletion | This work |
| pLAFR3 | Cosmid derivative of pLAFR1, IncP1, Tcr | 33 |
| pAX24 | pLAFR3 bearing all the xcp genes | 11 |
| pMMB67D-1867 | pMMB67HE bearing the xphA gene from the gateway system with V5 and His tags | G. Ball |
| pMMB-PAQA | pMMB190 bearing xphA-xqhA genes | This work |
| Mini CTX-lacZ | Tcr; self-proficient integration vector with Tet, Ω-FRT-attP-MCS, ori, int, and oriT | 18 |
| Mini CTX-pPA-lacZ | Mini CTX-lacZ bearing a transcriptional xphA-lacZ fusion | This work |