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Detection of the [2Fe-2S] cluster localized in subunit NqrF of the membrane-bound Na+-NQR by EPR spectroscopy. Membranes from the wild-type V. cholerae strain containing the Na+-NQR (solid line) and from the nqrC insertion mutant (dotted line) were mixed with 7.3 mM Na2-NADH prior to freezing. EPR conditions: microwave frequency, 9.652 GHz; modulation amplitude, 0.5 mT; microwave power, 2 mW; temperature, 40 K. Characteristic g values are indicated.
