TABLE 1.
Primers used in this study
| Primer | Sequence |
|---|---|
| PlinkRB | 5′-AAGGTCCATGCCTTCCTCC-3′/5′-TCTTTGTCACTATCTCGAAACCAA-3′ |
| PlinkBC | 5′-AGGTCCATGCCTTCCTCC-3′/5′-CCCGTGCTCATAGCCCTA-3′ |
| PlinkCC | 5′-CCGCCAAAGGGTTTAGTC-3′/5′-CGGGAGACAAGAAGAAACTC-3′ |
| ParsGSP1 | 5′-GAAGCTGGATGACCTTCTCG-3′ |
| ParsGSP2 | 5′-GAACGGGATGCCAAGATAGA-3′ |
| ParsGSP3 | 5′-CTTGACGTCCGGGTACAGAT-3′ |
| PdTanchor | 5′-GACCACGCGTATCGATGTCGACTTTTTTTTTTTTV-3′ |
| Panchor | 5′-GACCACGCGTATCGATGTCGAC-3′ |
| ParsC1 | 5′-GGTTCATGTTCGCCTCTGCC-3′/5′-GGCCGTCTCTTTCGCGCTG-3′ |
| ParsRBCC | 5′-CCATTAGCTTGTTGCTACACC-3′/5′-GCCAAGGTGCTAATGAAATGA-3′ |
| ParsC1RTa | 5′-GGTTCATGTTCGCCTCTGCC-3′ |
| ParsRRTa | 5′-CGCAGCACGTTCAAAACTC-3′ |
| ParsBRTa | 5′-GACATGACAGACACCGGCAA-3′ |
| ParsC2RTa | 5′-CACCGAATTTGTCACAACGC-3′ |
| ParsC3RTa | 5′-TCGATGGTCTTTGACTCTTGC-3′ |
| P16SRTa | 5′-CTGCTGGCACGGAGTTAGC-3′ |
| ParsC1RealTb | 5′-GTCAAAGCTGGTTTCCGAACTG-3′/5′-GGCTTTCGGCAGGAAAAAA-3′ |
| ParsRRealTb | 5′-TCACAGAAGCCTTAGCTTTGC-3′/5′-ATCCATTTTCCGCTCCTTCT-3′ |
| ParsBRealTb | 5′-ATCGCCATCGGCTTGATTC-3′/5′-GACAATGCGAGCACCTTGAA-3′ |
| ParsC2RealTb | 5′-ATTCCGCTGGTGTGAAAAAG-3′/5′-TCGATGGTCTTTGACTCTTGC-3′ |
| ParsC3RealTb | 5′-ACTCCTGTCGAAGCCAGATG-3′/5′-GGGTTTAGTCCGTGCTTTTTC-3′ |
| P16SRealTb | 5′-GGGTGAGTAACGCGTGGATT-3′/5′-AGCAGAGGCCCCCTTTACC-3′ |
a
Primers were generated manually based on the sequence at the end of each gene.
b
Primers were generated by Primer Express (ABI Prism).