TABLE 2.
Mean infection frequencies of wild-type and mutant strains of N. punctiforme estimated after 14 and 28 days of coculture with Blasia
| N. punctiforme strain (putative homologue)a | 14 days
|
28 days
|
||||
|---|---|---|---|---|---|---|
| No. of coculture replicates | Infection frequency (%)b
|
No. of coculture replicates | Infection frequency (%)b
|
|||
| Mean | SD | Mean | SD | |||
| Wild-type N. unctiforme | 28 | 12.03 | 7.8 | 32 | 15.95 | 12 |
| NpF0069 mutant (pilA) | 7 | 3.49 | 1.12 | 8 | 6.00 | 2.72 |
| NpF2507 mutant (pilT) | 23 | 6.00 | 4.35 | 18 | 14.13 | 8.35 |
| NpR0117 mutant (pilT) | 17 | 0.48 | 0.56 | 18 | 1.52 | 1.03 |
| NpR2800 mutant (pilD) | 20 | 0.68 | 0.4 | 20 | 0.39 | 0.37 |
a
The putative homologue of each ORF is indicated in parentheses.
b
Values are expressed as the mean number of infected auricles (the symbiotic cavities of Blasia) expressed as a percentage of the total number of auricles counted for each determination (a minimum of 400 auricles were counted for each determination).