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Purified IPMDHMj and combinations of large and small subunits of the hydrolyase apoenzymes. Lane 1, 8 μg His10MJ0720 (IPMDHMj); lane 2, 10 μg MJ1003/MJ1277; lane 3, 10 μg MJ1003/MJ1271; lane 4, 10 μg MJ0499/MJ1277; lane M, protein size markers (Bio-Rad). Proteins were separated by SDS-PAGE on a 15% acrylamide gel and stained with Coomassie blue.
