TABLE 3.
Kinetic parameters for IPMDH homologs
| Enzyme (reference) | β-Isopropylmalate
|
d-Malate
|
||
|---|---|---|---|---|
| Km (μM) | kcat/Km (M−1 s−1) | Km (μM) | kcat/Km (M−1 s−1) | |
| IPMDHMja | 24 ± 3 | 8.8 × 104 | 410 ± 40 | 1.4 × 104 |
| Sulfolobus sp. IPMDH (34) | 1.2 | 3.0 × 106 | 390 | 1.0 × 104 |
| P. putida TDH (37) | 14 | 1.1 × 104 | 60 | 2.2 × 105 |
| T. thermophilus IPMDH (9) | 17 | 1.4 × 104 | 1,510 | 7.3 × 103 |
a
Data from this work. Initial rates were determined by measuring an increase in absorbance at 340 nm due to NADH in reaction mixtures incubated at 60°C.