FIG. 1.

Motile strains form greater amounts of surface-adhered biofilm than nonmotile strains. (A) CV-stained L. monocytogenes biofilms formed on wells of a 96-well PVC plate. The first well has medium alone (Media), followed by the wild type (WT), flagellum-minus mutant (Fla⁻), paralyzed-flagellum mutant (Paralyzed), and nonglycosylated-flagellum mutant (Glyco⁻). (B) Graph of surface-adhered biofilm (quantified by measuring the OD₅₉₀ of solubilized CV after biofilm staining) from four independent experiments with seven replicates of each strain per experiment. Error bars represent standard errors of the means. Analysis using one-way ANOVA followed by Tukey's multiple comparison test (set at 5%) indicated that there was a statistically significant difference between the amounts of biofilm formed by motile and nonmotile strains on each of five days.