FIG. 1.

Validation of microarray results by qPCR. Three independent RNA samples were isolated from cultures of B. cereus strain ATCC 14579 (WT) and the sigB deletion mutant during mid-exponential growth at 30°C (ME) and after a heat shock to 42°C for 10 min (HS). The expression ratios between both strains of the indicated genes were measured using microarrays (closed squares) and qPCR (open circles). Small symbols indicate that no significant difference was measured for the expression levels of the target gene between the two strains.