TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype, phenotype, and/or property | Source or reference(s) |
|---|---|---|
| Strains | ||
| S. epidermidis O-47 | Biofilm positive | 15 |
| S. epidermidis hemB mutant | O-47 hemB::ermB SCV | This study |
| Complemented hemB mutant | O-47 hemB::ermB/pNA10 | This study |
| S. aureus SA113 | 8325, restriction deficient | ATCC 35556; 19 |
| S. carnosus TM 300 | Cloning host | |
| E. coli DH5α | supE44 ΔlacU169 (Φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 14 |
| E. coli GM161 | dam dcm | R. Brückner |
| E. coli TG1 | supE hsdΔ5 thi Δ(lac-proAB) F′ [traD36 proAB+lacIqlacZΔM15] | DSM 6056; 7 |
| Plasmids | ||
| pUC19 | Cloning vector | 48 |
| pEC4 | Composed of pUC19 and transposon Tn551 containing ermB erythromycin resistance cassette | 6 |
| pBT9 | E. coli-S. aureus shuttle vector | 49 |
| pCX19 | Derivate of pCX15 | 31, 47 |
| pNA1 | pUC19::hemB | This study |
| pNA2 | pUC19::hemB-ermB-hemB | This study |
| pNA3 | pBT9::hemB-ermB-hemB | This study |
| pNA10 | pCX19::hemB | This study |