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NADPH- and CoA-dependent S0 reductase activities in cellular fractions of P. furiosus. The cells were obtained from a maltose-grown culture prior to (white bars) and 1 h after (gray bars) the addition of S0. The fractions are cell extract (CE), cytoplasm (CT [supernatant after 1 h at 120,000 × g]), and membrane (M [pellet after 1 h 120,000 × g]). Elemental sulfur reductase activity is expressed as units/mg.
